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Image Search Results
Journal: Cell Proliferation
Article Title: p62 functions as an oncogene in colorectal cancer through inhibiting apoptosis and promoting cell proliferation by interacting with the vitamin D receptor
doi: 10.1111/cpr.12585
Figure Lengend Snippet: p62 shows tumour‐promoting ability in CRC cells. A, Western blotting of p62 in 6 human CRC cell lines and one normal human intestinal epithelial cell line. B, HCT116 was stably transduced with a p62‐overexpression lentiviral vector (p62‐OE), and SW480 was stably transduced with a p62‐knockdown lentiviral vector (p62‐KD). Accordingly, control groups were transduced with the corresponding lentiviral carrying an empty vector (EV). Overexpression or silencing of p62 was confirmed by RT‐qPCR (left panel) and Western blotting (right panel). C, Overexpression of p62 significantly promoted cell viability by CCK‐8 assay (left panel) compared to that of the control groups in HCT116. Knockdown of p62 significantly reduced cell viability by CCK‐8 assay (right panel) compared to that of the controls in SW480. D, Overexpression of p62 significantly increased colony numbers (left panel) compared to those of the control groups in HCT116. Knockdown of p62 significantly decreased colony numbers (right panel) compared to those of the controls in SW480. E, Apoptosis was analysed by flow cytometry. Knockdown of p62 in SW480 cells induced early apoptosis compared to the control cells. F, Expression of the cleaved form and the total level of caspase‐7 and PARP1 were detected by Western blotting. Bars represent the standard error of the mean ± SD from three independent experiments. *Represents Student's t test * P < 0.05, ** P < 0.01 and *** P < 0.001.
Article Snippet: The hU6‐MCS‐CMV‐EGFP knockdown lentiviral vector for SQSTM1 , the Ubi‐MCS‐3FLAG‐SV40‐puromycin overexpressing lentiviral vector for SQSTM1 and the
Techniques: Western Blot, Stable Transfection, Transduction, Over Expression, Plasmid Preparation, Quantitative RT-PCR, CCK-8 Assay, Flow Cytometry, Expressing